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rnase  (Thermo Fisher)


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    Thermo Fisher rnase
    Rnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnase+free/Water%2C+RNase%2C+DNase-free/pmc13234486-106-8-13
    Average 99 stars, based on 1 article reviews
    rnase - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: AGP-Ca 2+ binding is essential for pollen development and pollen tube growth in Arabidopsis thaliana
    Article Snippet: Total RNA was extracted using PureZol RNA Isolation Reagent (Bio-Rad) following the manufacturer’s protocol. .. One microgram of RNA was treated with DNase I, RNase-free (Thermo Fisher Scientific) according to the manufacturer’s instructions. cDNA was synthesised using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) with oligo(dT)18 primers, following the manufacturer’s guidelines, and diluted to 5 ng/μL in nuclease-free water (Sigma-Aldrich) for quantitative real-time PCR (qPCR) analysis. qPCR primers for GLCAT14A , GLCAT14B , and GLCAT14D were designed (Supplemental Table 5) and validated as described previously . .. Primer efficiency, correlation coefficient (R 2 ), and melting temperature were determined from a standard curve generated using a three-point tenfold dilution series of cDNA from WT inflorescences ( ). qPCR reactions were performed as reported previously using 2× SsoAdvanced Universal SYBR® Green Supermix (Bio-Rad) with three technical replicates per biological replicate and non-template controls on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad).

    Real-time Polymerase Chain Reaction:

    Article Title: AGP-Ca 2+ binding is essential for pollen development and pollen tube growth in Arabidopsis thaliana
    Article Snippet: Total RNA was extracted using PureZol RNA Isolation Reagent (Bio-Rad) following the manufacturer’s protocol. .. One microgram of RNA was treated with DNase I, RNase-free (Thermo Fisher Scientific) according to the manufacturer’s instructions. cDNA was synthesised using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) with oligo(dT)18 primers, following the manufacturer’s guidelines, and diluted to 5 ng/μL in nuclease-free water (Sigma-Aldrich) for quantitative real-time PCR (qPCR) analysis. qPCR primers for GLCAT14A , GLCAT14B , and GLCAT14D were designed (Supplemental Table 5) and validated as described previously . .. Primer efficiency, correlation coefficient (R 2 ), and melting temperature were determined from a standard curve generated using a three-point tenfold dilution series of cDNA from WT inflorescences ( ). qPCR reactions were performed as reported previously using 2× SsoAdvanced Universal SYBR® Green Supermix (Bio-Rad) with three technical replicates per biological replicate and non-template controls on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad).

    Reverse Transcription:

    Article Title: Characterization of adaptive evolution strains for the development of triclosan resistance in Agrobacterium tumefaciens C58
    Article Snippet: .. The RNA samples were treated with DNase I, RNase-free (Thermo Scientific), and reverse-transcribed using RevertAid Reverse Transcriptase (Thermo Scientific) with random hexamer primers (BioDesign, Thailand). ..

    Article Title: MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids.
    Article Snippet: New England BioLabs Cat# M0202M T4 polynucleotide kinase, 10 U/μl New England BioLabs Cat# M0201L RLT buffer Qiagen Cat# 79216 Ampicillin sodium salt Roth Cat# K029.2 Bovines serumalbumin (BSA) fraction V, NZ-origin Roth Cat# 8076.3 Deoxycholic acid sodium salt (DOC), 100 g Roth Cat# 3484.3 Dimethylsulfoxid (DMSO), 1l, p. a. Roth Cat# 4720.1 Ethidium bromide solution 1 % Roth Cat# 2218.1 Proteinase K Roth Cat# 7528.2 RNase A Roth Cat# 7156.1 Trichloroacetic acid solution, 100 g Roth Cat# 3744.3 Triton X-100 Roth Cat# 3051.4 16% Paraformaldehyde (Formaldehyde) Aqueous Solution, EM Grade Science Services Cat#E15710 CBL0137 HCl Selleckchem / Biozol Cat# SEL-S8483-5MG Trypsin, MS approved Serva Cat# 37286 AmershamTM Protran® Premium Western blotting membranes, nitrocellulose Sigma-Aldrich Cat# GE10600008 Doxycycline hyclate Sigma-Aldrich Cat# D9891 Duolink In Situ PLA Probe Anti-Mouse MINUS Sigma-Aldrich Cat# DUO92004 Duolink In Situ PLA Probe Anti-Rabbit PLUS Sigma-Aldrich Cat# DUO92002 Duolink In Situ Wash Buffers, Fluorescence Sigma-Aldrich Cat# DUO82049 Hoechst 33342 Sigma-Aldrich Cat# B2261-25MG Igepal CA-630 Sigma-Aldrich Cat# 18896 Penicillin-Streptomycin Solution stab. .. Sigma-Aldrich Cat# P4333 Phosphatase inhibitor cocktail #1 Sigma-Aldrich Cat# P5726 Phosphatase inhibitor cocktail #2 Sigma-Aldrich Cat# P0044 Polybrene Sigma-Aldrich Cat# H9268 Polyethylenimine, linear Sigma-Aldrich Cat# 764604 Protease inhibitor cocktail Sigma-Aldrich Cat# P8340 ReadyBlueTM Protein Gel Stain Sigma-Aldrich Cat# RSB-1L Tween-20 Sigma-Aldrich Cat# P1379 DMEM, high glucose, pyruvate Thermo Fischer Scientific Cat# 41966052 Lipofectamine RNAiMAX Transfection Reagent Thermo Fischer Scientific Cat#13778-150 Reagent RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fischer Scientific Cat#10777019 SuperScript III Reverse Transcriptase Thermo Fischer Scientific Cat#T18080044 AmbionTM RNase I, 100 U/μl Thermo Fisher Scientific Cat# AM2294 Dithiothreitol (DTT) Thermo Fisher Scientific Cat# 20291 DNase I, RNase-free (supplied with MnCl2) (1 U/μL) Thermo Fisher Scientific Cat# EN0525 Dynabead Protein A Thermo Fisher Scientific Cat# 10002D Dynabead Protein G Thermo Fisher Scientific Cat# 10004D Dynabeads MyOneTM Streptavidin T1 Thermo Fisher Scientific Cat# 65601 GeneRuler 1 kb Plus DNA-Ladder Thermo Fisher Scientific Cat# SM1332 (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e16, March 5, 2026 e3 Please cite this article in press as: Uhl et al., MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.019 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemiluminescent nucleic acid detection module Thermo Fisher Scientific 89880 RNA Clean & Concentrator-5 (DNase not Included) Zymo Research R1015 Q5 Site-Directed Mutagenesis Kit NEB E0554S NEBNext® UltraTM II Q5® Master Mix NEB M0544 L NEBuilder HiFi DNA Assembly Master Mix NEB E2621S GeneJET Gel extraction Kit Thermo Fisher Scientific K0692 T4 DNA Ligase (5U/μL) Thermo Fisher Scientific 26617 MaXtract High Density (200 x 1.5 mL) Qiagen 129046 Duolink In Situ Detection Reagents Orange Sigma-Aldrich Cat# DUO92007 Duolink In Situ Detection Reagents Green Sigma-Aldrich Cat# DUO92014 Deposited data Human reference genome GRCh37/hg19 Genome Reference Consortium http://grch37.ensembl.org/index.html Human reference genome GRCh38/hg38 Genome Reference Consortium http://grch38.ensembl.org/index.html Mouse reference genome mm39 Genome Reference Consortium http://www.ensembl.org/Mus_musculus/ Info/Index Raw and processed sequencing data This paper GEO: GSE281781 Experimental models: Cell lines

    Random Hexamer:

    Article Title: Characterization of adaptive evolution strains for the development of triclosan resistance in Agrobacterium tumefaciens C58
    Article Snippet: .. The RNA samples were treated with DNase I, RNase-free (Thermo Scientific), and reverse-transcribed using RevertAid Reverse Transcriptase (Thermo Scientific) with random hexamer primers (BioDesign, Thailand). ..

    Protease Inhibitor:

    Article Title: MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids.
    Article Snippet: New England BioLabs Cat# M0202M T4 polynucleotide kinase, 10 U/μl New England BioLabs Cat# M0201L RLT buffer Qiagen Cat# 79216 Ampicillin sodium salt Roth Cat# K029.2 Bovines serumalbumin (BSA) fraction V, NZ-origin Roth Cat# 8076.3 Deoxycholic acid sodium salt (DOC), 100 g Roth Cat# 3484.3 Dimethylsulfoxid (DMSO), 1l, p. a. Roth Cat# 4720.1 Ethidium bromide solution 1 % Roth Cat# 2218.1 Proteinase K Roth Cat# 7528.2 RNase A Roth Cat# 7156.1 Trichloroacetic acid solution, 100 g Roth Cat# 3744.3 Triton X-100 Roth Cat# 3051.4 16% Paraformaldehyde (Formaldehyde) Aqueous Solution, EM Grade Science Services Cat#E15710 CBL0137 HCl Selleckchem / Biozol Cat# SEL-S8483-5MG Trypsin, MS approved Serva Cat# 37286 AmershamTM Protran® Premium Western blotting membranes, nitrocellulose Sigma-Aldrich Cat# GE10600008 Doxycycline hyclate Sigma-Aldrich Cat# D9891 Duolink In Situ PLA Probe Anti-Mouse MINUS Sigma-Aldrich Cat# DUO92004 Duolink In Situ PLA Probe Anti-Rabbit PLUS Sigma-Aldrich Cat# DUO92002 Duolink In Situ Wash Buffers, Fluorescence Sigma-Aldrich Cat# DUO82049 Hoechst 33342 Sigma-Aldrich Cat# B2261-25MG Igepal CA-630 Sigma-Aldrich Cat# 18896 Penicillin-Streptomycin Solution stab. .. Sigma-Aldrich Cat# P4333 Phosphatase inhibitor cocktail #1 Sigma-Aldrich Cat# P5726 Phosphatase inhibitor cocktail #2 Sigma-Aldrich Cat# P0044 Polybrene Sigma-Aldrich Cat# H9268 Polyethylenimine, linear Sigma-Aldrich Cat# 764604 Protease inhibitor cocktail Sigma-Aldrich Cat# P8340 ReadyBlueTM Protein Gel Stain Sigma-Aldrich Cat# RSB-1L Tween-20 Sigma-Aldrich Cat# P1379 DMEM, high glucose, pyruvate Thermo Fischer Scientific Cat# 41966052 Lipofectamine RNAiMAX Transfection Reagent Thermo Fischer Scientific Cat#13778-150 Reagent RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fischer Scientific Cat#10777019 SuperScript III Reverse Transcriptase Thermo Fischer Scientific Cat#T18080044 AmbionTM RNase I, 100 U/μl Thermo Fisher Scientific Cat# AM2294 Dithiothreitol (DTT) Thermo Fisher Scientific Cat# 20291 DNase I, RNase-free (supplied with MnCl2) (1 U/μL) Thermo Fisher Scientific Cat# EN0525 Dynabead Protein A Thermo Fisher Scientific Cat# 10002D Dynabead Protein G Thermo Fisher Scientific Cat# 10004D Dynabeads MyOneTM Streptavidin T1 Thermo Fisher Scientific Cat# 65601 GeneRuler 1 kb Plus DNA-Ladder Thermo Fisher Scientific Cat# SM1332 (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e16, March 5, 2026 e3 Please cite this article in press as: Uhl et al., MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.019 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemiluminescent nucleic acid detection module Thermo Fisher Scientific 89880 RNA Clean & Concentrator-5 (DNase not Included) Zymo Research R1015 Q5 Site-Directed Mutagenesis Kit NEB E0554S NEBNext® UltraTM II Q5® Master Mix NEB M0544 L NEBuilder HiFi DNA Assembly Master Mix NEB E2621S GeneJET Gel extraction Kit Thermo Fisher Scientific K0692 T4 DNA Ligase (5U/μL) Thermo Fisher Scientific 26617 MaXtract High Density (200 x 1.5 mL) Qiagen 129046 Duolink In Situ Detection Reagents Orange Sigma-Aldrich Cat# DUO92007 Duolink In Situ Detection Reagents Green Sigma-Aldrich Cat# DUO92014 Deposited data Human reference genome GRCh37/hg19 Genome Reference Consortium http://grch37.ensembl.org/index.html Human reference genome GRCh38/hg38 Genome Reference Consortium http://grch38.ensembl.org/index.html Mouse reference genome mm39 Genome Reference Consortium http://www.ensembl.org/Mus_musculus/ Info/Index Raw and processed sequencing data This paper GEO: GSE281781 Experimental models: Cell lines

    Staining:

    Article Title: MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids.
    Article Snippet: New England BioLabs Cat# M0202M T4 polynucleotide kinase, 10 U/μl New England BioLabs Cat# M0201L RLT buffer Qiagen Cat# 79216 Ampicillin sodium salt Roth Cat# K029.2 Bovines serumalbumin (BSA) fraction V, NZ-origin Roth Cat# 8076.3 Deoxycholic acid sodium salt (DOC), 100 g Roth Cat# 3484.3 Dimethylsulfoxid (DMSO), 1l, p. a. Roth Cat# 4720.1 Ethidium bromide solution 1 % Roth Cat# 2218.1 Proteinase K Roth Cat# 7528.2 RNase A Roth Cat# 7156.1 Trichloroacetic acid solution, 100 g Roth Cat# 3744.3 Triton X-100 Roth Cat# 3051.4 16% Paraformaldehyde (Formaldehyde) Aqueous Solution, EM Grade Science Services Cat#E15710 CBL0137 HCl Selleckchem / Biozol Cat# SEL-S8483-5MG Trypsin, MS approved Serva Cat# 37286 AmershamTM Protran® Premium Western blotting membranes, nitrocellulose Sigma-Aldrich Cat# GE10600008 Doxycycline hyclate Sigma-Aldrich Cat# D9891 Duolink In Situ PLA Probe Anti-Mouse MINUS Sigma-Aldrich Cat# DUO92004 Duolink In Situ PLA Probe Anti-Rabbit PLUS Sigma-Aldrich Cat# DUO92002 Duolink In Situ Wash Buffers, Fluorescence Sigma-Aldrich Cat# DUO82049 Hoechst 33342 Sigma-Aldrich Cat# B2261-25MG Igepal CA-630 Sigma-Aldrich Cat# 18896 Penicillin-Streptomycin Solution stab. .. Sigma-Aldrich Cat# P4333 Phosphatase inhibitor cocktail #1 Sigma-Aldrich Cat# P5726 Phosphatase inhibitor cocktail #2 Sigma-Aldrich Cat# P0044 Polybrene Sigma-Aldrich Cat# H9268 Polyethylenimine, linear Sigma-Aldrich Cat# 764604 Protease inhibitor cocktail Sigma-Aldrich Cat# P8340 ReadyBlueTM Protein Gel Stain Sigma-Aldrich Cat# RSB-1L Tween-20 Sigma-Aldrich Cat# P1379 DMEM, high glucose, pyruvate Thermo Fischer Scientific Cat# 41966052 Lipofectamine RNAiMAX Transfection Reagent Thermo Fischer Scientific Cat#13778-150 Reagent RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fischer Scientific Cat#10777019 SuperScript III Reverse Transcriptase Thermo Fischer Scientific Cat#T18080044 AmbionTM RNase I, 100 U/μl Thermo Fisher Scientific Cat# AM2294 Dithiothreitol (DTT) Thermo Fisher Scientific Cat# 20291 DNase I, RNase-free (supplied with MnCl2) (1 U/μL) Thermo Fisher Scientific Cat# EN0525 Dynabead Protein A Thermo Fisher Scientific Cat# 10002D Dynabead Protein G Thermo Fisher Scientific Cat# 10004D Dynabeads MyOneTM Streptavidin T1 Thermo Fisher Scientific Cat# 65601 GeneRuler 1 kb Plus DNA-Ladder Thermo Fisher Scientific Cat# SM1332 (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e16, March 5, 2026 e3 Please cite this article in press as: Uhl et al., MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.019 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemiluminescent nucleic acid detection module Thermo Fisher Scientific 89880 RNA Clean & Concentrator-5 (DNase not Included) Zymo Research R1015 Q5 Site-Directed Mutagenesis Kit NEB E0554S NEBNext® UltraTM II Q5® Master Mix NEB M0544 L NEBuilder HiFi DNA Assembly Master Mix NEB E2621S GeneJET Gel extraction Kit Thermo Fisher Scientific K0692 T4 DNA Ligase (5U/μL) Thermo Fisher Scientific 26617 MaXtract High Density (200 x 1.5 mL) Qiagen 129046 Duolink In Situ Detection Reagents Orange Sigma-Aldrich Cat# DUO92007 Duolink In Situ Detection Reagents Green Sigma-Aldrich Cat# DUO92014 Deposited data Human reference genome GRCh37/hg19 Genome Reference Consortium http://grch37.ensembl.org/index.html Human reference genome GRCh38/hg38 Genome Reference Consortium http://grch38.ensembl.org/index.html Mouse reference genome mm39 Genome Reference Consortium http://www.ensembl.org/Mus_musculus/ Info/Index Raw and processed sequencing data This paper GEO: GSE281781 Experimental models: Cell lines

    Transfection:

    Article Title: MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids.
    Article Snippet: New England BioLabs Cat# M0202M T4 polynucleotide kinase, 10 U/μl New England BioLabs Cat# M0201L RLT buffer Qiagen Cat# 79216 Ampicillin sodium salt Roth Cat# K029.2 Bovines serumalbumin (BSA) fraction V, NZ-origin Roth Cat# 8076.3 Deoxycholic acid sodium salt (DOC), 100 g Roth Cat# 3484.3 Dimethylsulfoxid (DMSO), 1l, p. a. Roth Cat# 4720.1 Ethidium bromide solution 1 % Roth Cat# 2218.1 Proteinase K Roth Cat# 7528.2 RNase A Roth Cat# 7156.1 Trichloroacetic acid solution, 100 g Roth Cat# 3744.3 Triton X-100 Roth Cat# 3051.4 16% Paraformaldehyde (Formaldehyde) Aqueous Solution, EM Grade Science Services Cat#E15710 CBL0137 HCl Selleckchem / Biozol Cat# SEL-S8483-5MG Trypsin, MS approved Serva Cat# 37286 AmershamTM Protran® Premium Western blotting membranes, nitrocellulose Sigma-Aldrich Cat# GE10600008 Doxycycline hyclate Sigma-Aldrich Cat# D9891 Duolink In Situ PLA Probe Anti-Mouse MINUS Sigma-Aldrich Cat# DUO92004 Duolink In Situ PLA Probe Anti-Rabbit PLUS Sigma-Aldrich Cat# DUO92002 Duolink In Situ Wash Buffers, Fluorescence Sigma-Aldrich Cat# DUO82049 Hoechst 33342 Sigma-Aldrich Cat# B2261-25MG Igepal CA-630 Sigma-Aldrich Cat# 18896 Penicillin-Streptomycin Solution stab. .. Sigma-Aldrich Cat# P4333 Phosphatase inhibitor cocktail #1 Sigma-Aldrich Cat# P5726 Phosphatase inhibitor cocktail #2 Sigma-Aldrich Cat# P0044 Polybrene Sigma-Aldrich Cat# H9268 Polyethylenimine, linear Sigma-Aldrich Cat# 764604 Protease inhibitor cocktail Sigma-Aldrich Cat# P8340 ReadyBlueTM Protein Gel Stain Sigma-Aldrich Cat# RSB-1L Tween-20 Sigma-Aldrich Cat# P1379 DMEM, high glucose, pyruvate Thermo Fischer Scientific Cat# 41966052 Lipofectamine RNAiMAX Transfection Reagent Thermo Fischer Scientific Cat#13778-150 Reagent RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fischer Scientific Cat#10777019 SuperScript III Reverse Transcriptase Thermo Fischer Scientific Cat#T18080044 AmbionTM RNase I, 100 U/μl Thermo Fisher Scientific Cat# AM2294 Dithiothreitol (DTT) Thermo Fisher Scientific Cat# 20291 DNase I, RNase-free (supplied with MnCl2) (1 U/μL) Thermo Fisher Scientific Cat# EN0525 Dynabead Protein A Thermo Fisher Scientific Cat# 10002D Dynabead Protein G Thermo Fisher Scientific Cat# 10004D Dynabeads MyOneTM Streptavidin T1 Thermo Fisher Scientific Cat# 65601 GeneRuler 1 kb Plus DNA-Ladder Thermo Fisher Scientific Cat# SM1332 (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e16, March 5, 2026 e3 Please cite this article in press as: Uhl et al., MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.019 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemiluminescent nucleic acid detection module Thermo Fisher Scientific 89880 RNA Clean & Concentrator-5 (DNase not Included) Zymo Research R1015 Q5 Site-Directed Mutagenesis Kit NEB E0554S NEBNext® UltraTM II Q5® Master Mix NEB M0544 L NEBuilder HiFi DNA Assembly Master Mix NEB E2621S GeneJET Gel extraction Kit Thermo Fisher Scientific K0692 T4 DNA Ligase (5U/μL) Thermo Fisher Scientific 26617 MaXtract High Density (200 x 1.5 mL) Qiagen 129046 Duolink In Situ Detection Reagents Orange Sigma-Aldrich Cat# DUO92007 Duolink In Situ Detection Reagents Green Sigma-Aldrich Cat# DUO92014 Deposited data Human reference genome GRCh37/hg19 Genome Reference Consortium http://grch37.ensembl.org/index.html Human reference genome GRCh38/hg38 Genome Reference Consortium http://grch38.ensembl.org/index.html Mouse reference genome mm39 Genome Reference Consortium http://www.ensembl.org/Mus_musculus/ Info/Index Raw and processed sequencing data This paper GEO: GSE281781 Experimental models: Cell lines

    Recombinant:

    Article Title: MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids.
    Article Snippet: New England BioLabs Cat# M0202M T4 polynucleotide kinase, 10 U/μl New England BioLabs Cat# M0201L RLT buffer Qiagen Cat# 79216 Ampicillin sodium salt Roth Cat# K029.2 Bovines serumalbumin (BSA) fraction V, NZ-origin Roth Cat# 8076.3 Deoxycholic acid sodium salt (DOC), 100 g Roth Cat# 3484.3 Dimethylsulfoxid (DMSO), 1l, p. a. Roth Cat# 4720.1 Ethidium bromide solution 1 % Roth Cat# 2218.1 Proteinase K Roth Cat# 7528.2 RNase A Roth Cat# 7156.1 Trichloroacetic acid solution, 100 g Roth Cat# 3744.3 Triton X-100 Roth Cat# 3051.4 16% Paraformaldehyde (Formaldehyde) Aqueous Solution, EM Grade Science Services Cat#E15710 CBL0137 HCl Selleckchem / Biozol Cat# SEL-S8483-5MG Trypsin, MS approved Serva Cat# 37286 AmershamTM Protran® Premium Western blotting membranes, nitrocellulose Sigma-Aldrich Cat# GE10600008 Doxycycline hyclate Sigma-Aldrich Cat# D9891 Duolink In Situ PLA Probe Anti-Mouse MINUS Sigma-Aldrich Cat# DUO92004 Duolink In Situ PLA Probe Anti-Rabbit PLUS Sigma-Aldrich Cat# DUO92002 Duolink In Situ Wash Buffers, Fluorescence Sigma-Aldrich Cat# DUO82049 Hoechst 33342 Sigma-Aldrich Cat# B2261-25MG Igepal CA-630 Sigma-Aldrich Cat# 18896 Penicillin-Streptomycin Solution stab. .. Sigma-Aldrich Cat# P4333 Phosphatase inhibitor cocktail #1 Sigma-Aldrich Cat# P5726 Phosphatase inhibitor cocktail #2 Sigma-Aldrich Cat# P0044 Polybrene Sigma-Aldrich Cat# H9268 Polyethylenimine, linear Sigma-Aldrich Cat# 764604 Protease inhibitor cocktail Sigma-Aldrich Cat# P8340 ReadyBlueTM Protein Gel Stain Sigma-Aldrich Cat# RSB-1L Tween-20 Sigma-Aldrich Cat# P1379 DMEM, high glucose, pyruvate Thermo Fischer Scientific Cat# 41966052 Lipofectamine RNAiMAX Transfection Reagent Thermo Fischer Scientific Cat#13778-150 Reagent RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fischer Scientific Cat#10777019 SuperScript III Reverse Transcriptase Thermo Fischer Scientific Cat#T18080044 AmbionTM RNase I, 100 U/μl Thermo Fisher Scientific Cat# AM2294 Dithiothreitol (DTT) Thermo Fisher Scientific Cat# 20291 DNase I, RNase-free (supplied with MnCl2) (1 U/μL) Thermo Fisher Scientific Cat# EN0525 Dynabead Protein A Thermo Fisher Scientific Cat# 10002D Dynabead Protein G Thermo Fisher Scientific Cat# 10004D Dynabeads MyOneTM Streptavidin T1 Thermo Fisher Scientific Cat# 65601 GeneRuler 1 kb Plus DNA-Ladder Thermo Fisher Scientific Cat# SM1332 (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e16, March 5, 2026 e3 Please cite this article in press as: Uhl et al., MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.019 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemiluminescent nucleic acid detection module Thermo Fisher Scientific 89880 RNA Clean & Concentrator-5 (DNase not Included) Zymo Research R1015 Q5 Site-Directed Mutagenesis Kit NEB E0554S NEBNext® UltraTM II Q5® Master Mix NEB M0544 L NEBuilder HiFi DNA Assembly Master Mix NEB E2621S GeneJET Gel extraction Kit Thermo Fisher Scientific K0692 T4 DNA Ligase (5U/μL) Thermo Fisher Scientific 26617 MaXtract High Density (200 x 1.5 mL) Qiagen 129046 Duolink In Situ Detection Reagents Orange Sigma-Aldrich Cat# DUO92007 Duolink In Situ Detection Reagents Green Sigma-Aldrich Cat# DUO92014 Deposited data Human reference genome GRCh37/hg19 Genome Reference Consortium http://grch37.ensembl.org/index.html Human reference genome GRCh38/hg38 Genome Reference Consortium http://grch38.ensembl.org/index.html Mouse reference genome mm39 Genome Reference Consortium http://www.ensembl.org/Mus_musculus/ Info/Index Raw and processed sequencing data This paper GEO: GSE281781 Experimental models: Cell lines

    Binding Assay:

    Article Title: MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids.
    Article Snippet: New England BioLabs Cat# M0202M T4 polynucleotide kinase, 10 U/μl New England BioLabs Cat# M0201L RLT buffer Qiagen Cat# 79216 Ampicillin sodium salt Roth Cat# K029.2 Bovines serumalbumin (BSA) fraction V, NZ-origin Roth Cat# 8076.3 Deoxycholic acid sodium salt (DOC), 100 g Roth Cat# 3484.3 Dimethylsulfoxid (DMSO), 1l, p. a. Roth Cat# 4720.1 Ethidium bromide solution 1 % Roth Cat# 2218.1 Proteinase K Roth Cat# 7528.2 RNase A Roth Cat# 7156.1 Trichloroacetic acid solution, 100 g Roth Cat# 3744.3 Triton X-100 Roth Cat# 3051.4 16% Paraformaldehyde (Formaldehyde) Aqueous Solution, EM Grade Science Services Cat#E15710 CBL0137 HCl Selleckchem / Biozol Cat# SEL-S8483-5MG Trypsin, MS approved Serva Cat# 37286 AmershamTM Protran® Premium Western blotting membranes, nitrocellulose Sigma-Aldrich Cat# GE10600008 Doxycycline hyclate Sigma-Aldrich Cat# D9891 Duolink In Situ PLA Probe Anti-Mouse MINUS Sigma-Aldrich Cat# DUO92004 Duolink In Situ PLA Probe Anti-Rabbit PLUS Sigma-Aldrich Cat# DUO92002 Duolink In Situ Wash Buffers, Fluorescence Sigma-Aldrich Cat# DUO82049 Hoechst 33342 Sigma-Aldrich Cat# B2261-25MG Igepal CA-630 Sigma-Aldrich Cat# 18896 Penicillin-Streptomycin Solution stab. .. Sigma-Aldrich Cat# P4333 Phosphatase inhibitor cocktail #1 Sigma-Aldrich Cat# P5726 Phosphatase inhibitor cocktail #2 Sigma-Aldrich Cat# P0044 Polybrene Sigma-Aldrich Cat# H9268 Polyethylenimine, linear Sigma-Aldrich Cat# 764604 Protease inhibitor cocktail Sigma-Aldrich Cat# P8340 ReadyBlueTM Protein Gel Stain Sigma-Aldrich Cat# RSB-1L Tween-20 Sigma-Aldrich Cat# P1379 DMEM, high glucose, pyruvate Thermo Fischer Scientific Cat# 41966052 Lipofectamine RNAiMAX Transfection Reagent Thermo Fischer Scientific Cat#13778-150 Reagent RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fischer Scientific Cat#10777019 SuperScript III Reverse Transcriptase Thermo Fischer Scientific Cat#T18080044 AmbionTM RNase I, 100 U/μl Thermo Fisher Scientific Cat# AM2294 Dithiothreitol (DTT) Thermo Fisher Scientific Cat# 20291 DNase I, RNase-free (supplied with MnCl2) (1 U/μL) Thermo Fisher Scientific Cat# EN0525 Dynabead Protein A Thermo Fisher Scientific Cat# 10002D Dynabead Protein G Thermo Fisher Scientific Cat# 10004D Dynabeads MyOneTM Streptavidin T1 Thermo Fisher Scientific Cat# 65601 GeneRuler 1 kb Plus DNA-Ladder Thermo Fisher Scientific Cat# SM1332 (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e16, March 5, 2026 e3 Please cite this article in press as: Uhl et al., MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.019 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemiluminescent nucleic acid detection module Thermo Fisher Scientific 89880 RNA Clean & Concentrator-5 (DNase not Included) Zymo Research R1015 Q5 Site-Directed Mutagenesis Kit NEB E0554S NEBNext® UltraTM II Q5® Master Mix NEB M0544 L NEBuilder HiFi DNA Assembly Master Mix NEB E2621S GeneJET Gel extraction Kit Thermo Fisher Scientific K0692 T4 DNA Ligase (5U/μL) Thermo Fisher Scientific 26617 MaXtract High Density (200 x 1.5 mL) Qiagen 129046 Duolink In Situ Detection Reagents Orange Sigma-Aldrich Cat# DUO92007 Duolink In Situ Detection Reagents Green Sigma-Aldrich Cat# DUO92014 Deposited data Human reference genome GRCh37/hg19 Genome Reference Consortium http://grch37.ensembl.org/index.html Human reference genome GRCh38/hg38 Genome Reference Consortium http://grch38.ensembl.org/index.html Mouse reference genome mm39 Genome Reference Consortium http://www.ensembl.org/Mus_musculus/ Info/Index Raw and processed sequencing data This paper GEO: GSE281781 Experimental models: Cell lines



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    ethylenediaminetetraacetic acid trypsin edta trypsin  (Thermo Fisher)
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    Thermo Fisher ethylenediaminetetraacetic acid trypsin edta trypsin
    Ethylenediaminetetraacetic Acid Trypsin Edta Trypsin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 1 article reviews
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    chloroform  (Thermo Fisher)
    97
    Thermo Fisher chloroform
    Chloroform, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 1 article reviews
    chloroform - by Bioz Stars, 2026-09
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    sodium dodecyl sulfate sds  (Thermo Fisher)
    94
    Thermo Fisher sodium dodecyl sulfate sds
    Sodium Dodecyl Sulfate Sds, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ssc buffer rnase free  (Thermo Fisher)
    97
    Thermo Fisher ssc buffer rnase free
    Ssc Buffer Rnase Free, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ultrapuretm distilled water dnase rnase free  (Thermo Fisher)
    99
    Thermo Fisher ultrapuretm distilled water dnase rnase free
    Ultrapuretm Distilled Water Dnase Rnase Free, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    water bath  (Thermo Fisher)
    99
    Thermo Fisher water bath
    Water Bath, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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